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DNA Fingerprinting
"Who Done It?"
Introduction:
This is a simulation of the process of DNA
fingerprinting. In this exercise, students will simulate:
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preparation of restriction fragments from samples of DNA, and |
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separation of these fragments by gel electrophoresis. |
These processes yield evidence regarding a crime, and students are
asked to use the results of their simulated gel separations to infer
which suspect's blood was left at the scene of the crime. News
reports have publicized numerous crime events involving the use of
DNA evidence. In spite of the publicity, much of the underlying
science is not well understood by most people. As you will see, this
is not a simple activity, but includes several advanced concepts.
It assumes prior knowledge of the structure of DNA and its components,
as well as some understanding of the enzymatic function of proteins.
Finally, interpretation of results requires a careful analysis of some
rules of logic.
Concepts in this Activity:
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The use of restriction enzymes to prepare DNA fragments. |
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The use of gel electrophoresis to separate molecules of
different sizes. |
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The meanings of the terms "sticky ends" and "blunt ends." |
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The reasons why multiple tests, using different restriction
enzymes, are needed for DNA evidence. |
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The logic of scientific procedure (hypotheses may be rejected,
but they may not be proven). |
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The use of statistical analysis to estimate the probability that
a conclusion is correct. |
This Course Component in PDF
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Please download Adobe Acrobat® Reader
to view our PDF documents. |
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Appendices |
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Download
the Whole Course Component: DNA Fingerprinting (1,328 KB)
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Blount.
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